HOW CLONING WORKS?: Cloned embryos were produced in TUBITAK MRC, Genetic Engineering and Biotechnology Institute, Animal Genetics and Reproduction Biology Laboratory by using tissue sample of an Anatolian Grey Bull located in The Faculty of Veterinary Medicine, Uludag University, and for Ece, Ecem, Nilufer and Kiraz the tissue sample of a female cattle located in Marmara Region. First, the cells were isolated from the tissue, they are cultured and then the proliferated cells were cryopreserved in the cellbank. In order to test the cell bank, some of the cells were used as DNA source in the "Cloning of Anatolian Native Cattle Breeds" project. For this reason, in vitro matured oocytes were enucleated and the nucleus of somatic cells were transferred into enucleated mature oocytes by injection. After 7-day culture, when clon embryos reached blastocyst stage, they were transferred to the farm of The Faculty of Veterinary Medicine, Istanbul University and Uludag University in a portable incubator. Then, they were implanted into carrier mothers. In this respect, cloned Anatolian Grey embryos, which were sent to The Faculty of Veterinary Medicine, Istanbul University and Uludag University, were eventually developed into a healthy fetus and the male calf, Efe was born, then two female calves Ece & Ecem were born in Istanbul University, after a while two female calves Nilufer and Kiraz were born in Uludag University. In this project, more than 250 cloned embryos were produced, some of which were freezed and cryopreserved, some of which were transferred either to Istanbul University or to Uludag University for implanting into carrier mothers to be born.
Project Coordinator: |
Assoc.Prof.Dr.Sezen Arat TUBITAK MRC Genetic Engineering and Biotechnology Institute |
Instutitions that embryo transfer has been made: |
Istanbul and Uludag University, Faculty of Veterinary Medicine |
Grant Funding Institution: |
TUBITAK-TOVAG |
Aim of the Project: |
Provide a sustainable management system to prevent the extinction of Turkish breeds. |
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BT-Boz-TR16503202(6355)E Anatolian Grey Bull -the source of DNA- located in Uludag University |
Anatolian Grey Calf Reproduced by TUBITAK MRC EFE |
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 Anatolian Grey Cow -the source of DNA- obtained from Marmara Region (Yalova) (5 years old) |
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Anatolian Grey Calves Reproduced by TUBITAK MRC ECE & ECEM |
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Anatolian Grey Calves Reproduced by TUBITAK MRC NILUFER & KIRAZ |
General information about the project
Photo Album